Chemiluminescence Determination of 6-Mercaptopurine in the Luminol-Potassium Ferricyanide System
WANG Tao1, XUE Bing-chun1, LIU Er-bao1,2*
1. College of Chemistry and Material Science, Shanxi Normal University, Linfen 041000，China 2. Laboratory of Water Resources and Water Environment, Tianjin Normal University, Tianjin 300074, China
Abstract：A novel chemiluminescence (CL) method to determine 6-mercaptopurine (6-MP) is described, based on the catalytic effect of 6-MP on the CL reaction of luminol with K3Fe(C-N)6 in a flow system. Under optimal experimental conditions, the calibration graph is linear in the range of 1.02×10-7 to 1.02×10-9 mol·L-1 6-MP. The linear regression equation was Y=36.315c+140.72, where Y is the relative emission intensity and c is the concentration of 6-MP expressed in 10-8 mol·L-1 and the correlation coefficient is 0.998 2 with the detection limits of 6.33×10-10 mol·L-1(S/N=3). CL peak-height precision is 3.54% of RSD(n=12) for a level of 2.0×10-9 mol·L-1. This method satisfactorily provides a fast, simple, sensible and favorable technique for the determination of 6-MP in synthesized samples in implementation. Moreover, the possible mechanism of CL enhancement was briefly discussed. Obviously, the reaction was related to the formation of free radicals. Thus, the CL emission could preliminarily be attributed to the process of energy transfer due to O2. radical and ·OH radical resulting from the oxidation product of 6-MP by K3Fe(CN)6, which acts as an active intermediate that reacts with luminol, leading to the formation of excited luminol molecule, which gives out an emission when it returns to its ground state.
王 滔1, 薛冰纯1,刘二保1,2*. 化学发光法测定巯嘌呤的含量[J]. 光谱学与光谱分析, 2008, 28(05): 1026-1029.
WANG Tao1, XUE Bing-chun1, LIU Er-bao1,2*. Chemiluminescence Determination of 6-Mercaptopurine in the Luminol-Potassium Ferricyanide System. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(05): 1026-1029.
